• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
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  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    Device and method of photonic sensing comprising elements of bi-knowledge with conformational change. The present invention relates to a device and a method of photonic sensing for the detection of analytes (12) comprising a photonic structure (9) as a transduction element and biorecognition elements (10), related to analytes (12) a detect; said biorecognition elements (10) being able to undergo a conformational change when in the presence of the analytes (12) and having fixed particles (11) of a larger size than the analytes (12), of the order of nanometers and/or a refractive index greater than 1.3. (Machine-translation by Google Translate, not legally binding)
    公开号:ES2612550A1
    申请号:ES201631409
    申请日:2016-11-04
    公开日:2017-05-17
    发明作者:María José BAÑULS POLO；Jaime García Rupérez；Ángel Maquieira Catalá；Javier Martí Sendra
    申请人:Universidad Politecnica de Valencia；
    IPC主号:
    专利说明:

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    transduction shown in figure 3c.
    Figure 6 schematically describes a preferred embodiment of the present invention. In this preferred embodiment, the biorecognition elements based on molecular beacons attached to particles of large size and / or high index are immobilized on the surface of a sensing structure based on the phenomenon of photonic prohibited band. DESCRIPTION OF THE INVENTION
    A first aspect of the invention relates to a photonic sensing device for the detection of analytes comprising a transduction element in the form of a photonic structure and biorecognition elements related to the analytes to be detected. Said photonic sensing device is characterized in that the biorecognition elements undergo a conformational change when they are in the presence of the analyte to be detected and because said biorecognition elements have particles larger than the analytes to be detected, of the order of nanometers, and / or refractive index values greater than 1.3.
    The photonic sensing device according to the invention allows the detection of analytes with high sensitivity, through the use of biorecognition elements that undergo a conformational change (ie, a change in their spatial arrangement) upon detection of the desired analyte, so that This conformational change causes the displacement of particles fixed to the biorecognition elements themselves.
    The development of said photonic sensing device takes advantage of the physical properties of photon sensing structures based on spectral displacement. In these photonic sensing structures, their spectral response in transmission or reflection will depend on the dielectric configuration of the materials that make up the structure used, so any variation in that dielectric composition will result in a variation of the spectral response, which it will usually translate itself into a spectral shift. Therefore, by monitoring the frequency (or wavelength) displacement experienced by the spectral response of the photonic sensing structure, we will be able to detect the presence (and quantity) of the analytes of interest. The conformational change that occurs in the biorecognition elements due to the interaction with the analyte, causes the particles with a high refractive index (greater than
    eleven
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    transmission to the prohibited photonic band (2) and vice versa (3). Figure 1b shows the state in which the analyte sensing events have occurred. The detection of the analyte causes a variation of the refractive index in the photonic structure, in turn causing a shift in the transmission spectrum (Y) of it with respect to that shown in Figure 1a. In Figure 1b it can be seen how there has been a displacement of the photonic prohibited band and therefore a change in the position of the edges at which the transmission band is passed to the prohibited photonic band and vice versa, passing from the position (2) to (4) and of the
    (3) to (5) respectively. Figure 1c shows the evolution of the spectral position of the spectral characteristic being monitored (Y ') as a function of time (X'), which in this example could be the evolution of the position of the edge at which from the transmission band to the photonic prohibited band ((2) in figures 1a and 1b) or from the edge at which the photonic prohibited band is passed to the transmission band ((3) in figures 1a and 1b ). In this evolution, it can be seen how the monitored spectral characteristic remains at a certain wavelength (or frequency) (6), so that when sensing events occur, a shift in the position of the spectral characteristic (7) will occur until arrive at a maximum displacement position (8) determined by the specific experiment being carried out.
    The displacement experienced by the spectral response of a photonic sensing structure, shown in (7) in Figure 1c, will be determined, among other factors, by the concentration and by the molecular mass of the analyte to be detected. Thus, in the devices of the prior art, those situations in which there is a very low concentration of the analyte to be detected or those in which the molecular mass of the analyte is very reduced, will cause extremely reduced spectral shifts and that will be difficult to detect. by the sensing element.
    The photonic sensing device according to the invention allows, on the contrary, to amplify the spectral shift produced by the presence of analyte, improving the sensitivity in the photonic sensing.
    The principle of operation of a photonic sensing device according to the present invention is described schematically in Figure 2.
    Said photonic sensing device according to the invention comprises, as shown in
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    photonic sensing device, which will translate, in this specific embodiment of the invention, into an amplification factor of about 2 orders of magnitude on the sensing effect.
    5 This level of amplification of the sensing effect will be dependent on various parameters such as the type of analyte to be detected (ie, for light analytes, the effect of amplification by the conformational change may be higher), the refractive index and the size of the particle fixed to the biorecognition element, the position at which the particle (10) is fixed to the biorecognition element or the magnitude of the displacement of the
    10 particle (10) that is produced by the conformational change experienced, among others.
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    权利要求:
    Claims (1)
    [1]
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    同族专利:
    公开号 | 公开日
    WO2018083365A1|2018-05-11|
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    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    US20140113359A1|2006-10-23|2014-04-24|Banpil Photonics, Inc.|Highsensitivitysensordevice and manufacturing thereof|
    法律状态:
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    优先权:
    申请号 | 申请日 | 专利标题
    ES201631409A|ES2612550B2|2016-11-04|2016-11-04|DEVICE AND METHOD OF PHOTONIC SENSING THAT INCLUDE ELEMENTS OF WELFARE WITH CONFORMATIONAL CHANGE|ES201631409A| ES2612550B2|2016-11-04|2016-11-04|DEVICE AND METHOD OF PHOTONIC SENSING THAT INCLUDE ELEMENTS OF WELFARE WITH CONFORMATIONAL CHANGE|
    PCT/ES2017/070731| WO2018083365A1|2016-11-04|2017-11-03|Device and method for photonic sensing comprising elements for bio-recognition with a conformational change|
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